› 4v46

X-ray diffraction
3.3Å resolution

Crystal structure of the BAFF-BAFF-R complex

Released:
DOI: 10.2210/pdb4v46/pdb
PDB entry 4v46 coloured by chain, front view.
PDB entry 4v46 coloured by chain, side view.
PDB entry 4v46 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the BAFF-BAFF-R complex and its implications for receptor activation.
Kim HM, Yu KS, Lee ME, Shin DR, Kim YS, Paik SG, Yoo OJ, Lee H, Lee JO
Nat Struct Biol 10 342-8 (2003)
PMID: 12715002

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 120-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188728 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tumor necrosis factor ligand superfamily member 13b, soluble form Chains: A0, A1, A2, A3, A4, A5, A6, A7, A8, A9, AA, AB, AC, AD, AE, AF, AG, AH, AI, AJ, AK, AL, AM, AN, AO, AP, AQ, AR, AS, AT, AU, AV, AW, AX, AY, AZ, Aa, Ab, Ac, Ad, Ae, Af, Ag, Ah, Ai, Aj, Ak, Al, Am, An, Ao, Ap, Aq, Ar, As, At, Au, Av, Aw, Ax
Molecule details ›
Chains: A0, A1, A2, A3, A4, A5, A6, A7, A8, A9, AA, AB, AC, AD, AE, AF, AG, AH, AI, AJ, AK, AL, AM, AN, AO, AP, AQ, AR, AS, AT, AU, AV, AW, AX, AY, AZ, Aa, Ab, Ac, Ad, Ae, Af, Ag, Ah, Ai, Aj, Ak, Al, Am, An, Ao, Ap, Aq, Ar, As, At, Au, Av, Aw, Ax
Length: 148 amino acids
Theoretical weight: 16.7 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9Y275 (Residues: 138-285; Coverage: 52%)
Gene names: BAFF, BLYS, TALL1, TNFSF13B, TNFSF20, UNQ401/PRO738, ZTNF4
Sequence domains: TNF(Tumour Necrosis Factor) family
Tumor necrosis factor receptor superfamily member 13C Chains: B0, B1, B2, B3, B4, B5, B6, B7, B8, B9, BA, BB, BC, BD, BE, BF, BG, BH, BI, BJ, BK, BL, BM, BN, BO, BP, BQ, BR, BS, BT, BU, BV, BW, BX, BY, BZ, Ba, Bb, Bc, Bd, Be, Bf, Bg, Bh, Bi, Bj, Bk, Bl, Bm, Bn, Bo, Bp, Bq, Br, Bs, Bt, Bu, Bv, Bw, Bx
Molecule details ›
Chains: B0, B1, B2, B3, B4, B5, B6, B7, B8, B9, BA, BB, BC, BD, BE, BF, BG, BH, BI, BJ, BK, BL, BM, BN, BO, BP, BQ, BR, BS, BT, BU, BV, BW, BX, BY, BZ, Ba, Bb, Bc, Bd, Be, Bf, Bg, Bh, Bi, Bj, Bk, Bl, Bm, Bn, Bo, Bp, Bq, Br, Bs, Bt, Bu, Bv, Bw, Bx
Length: 63 amino acids
Theoretical weight: 6.73 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q96RJ3 (Residues: 1-63; Coverage: 34%)
Gene names: BAFFR, BR3, TNFRSF13C
Sequence domains: BAFF-R, TALL-1 binding

Ligands and Environments

1 bound ligand:
Ligand MG 40 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21
Unit cell:
a: 175.5Å b: 194.8Å c: 274.4Å
α: 90° β: 93.3° γ: 90°
R-values:
R R work R free
0.218 0.218 0.226
Expression system: Spodoptera frugiperda

Quick links

Citations

19 review citations

BAFF, APRIL and their receptors: structure, function and signaling.
Bossen et al. (2006)
18 more

5 mentions without citation

Stoichiometry of Heteromeric BAFF and APRIL Cytokines Dictates Their Receptor Binding and Signaling Properties.
Schuepbach-Mallepell et al. (2015)
4 more