4v4j

X-ray diffraction
3.83Å resolution

Interactions and Dynamics of the Shine-Dalgarno Helix in the 70S Ribosome.

Released:
Model geometry
Fit model/data
Source organism: Thermus thermophilus HB27

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 54-mer (preferred)
PDBe Complex ID:
PDB-CPX-293940 (preferred)
Entry contents:
48 distinct polypeptide molecules
6 distinct RNA molecules
Macromolecules (54 distinct):
Large ribosomal subunit protein uL1 Chain: A
Molecule details ›
Chain: A
Length: 229 amino acids
Theoretical weight: 24.87 KDa
Source organism: Thermus thermophilus HB27
UniProt:
  • Canonical: Q5SLP7 (Residues: 1-229; Coverage: 100%)
Gene names: TTHA0246, rplA
Sequence domains: Ribosomal protein L1p/L10e family
Large ribosomal subunit protein uL2 Chain: B
Molecule details ›
Chain: B
Length: 276 amino acids
Theoretical weight: 30.53 KDa
Source organism: Thermus thermophilus HB27
UniProt:
  • Canonical: P60405 (Residues: 1-276; Coverage: 100%)
Gene names: TTHA1689, rplB
Sequence domains:
Large ribosomal subunit protein uL3 Chain: C
Large ribosomal subunit protein uL4 Chain: D
Large ribosomal subunit protein uL5 Chain: E
Large ribosomal subunit protein uL6 Chain: F
Large ribosomal subunit protein bL9 Chain: G
Large ribosomal subunit protein uL13 Chain: H
Large ribosomal subunit protein uL14 Chain: I
Large ribosomal subunit protein uL15 Chain: J
Large ribosomal subunit protein uL16 Chain: K
Large ribosomal subunit protein bL17 Chain: L
Large ribosomal subunit protein uL18 Chain: M
Large ribosomal subunit protein bL19 Chain: N
Large ribosomal subunit protein bL20 Chain: O
Large ribosomal subunit protein bL21 Chain: P
Large ribosomal subunit protein uL22 Chain: Q
Large ribosomal subunit protein uL23 Chain: R
Large ribosomal subunit protein uL24 Chain: S
Large ribosomal subunit protein bL25 Chain: T
Large ribosomal subunit protein bL27 Chain: U
Large ribosomal subunit protein bL28 Chain: V
Large ribosomal subunit protein uL29 Chain: W
Large ribosomal subunit protein uL30 Chain: X
Large ribosomal subunit protein bL32 Chain: Y
Large ribosomal subunit protein bL34 Chain: Z
Large ribosomal subunit protein bL35 Chain: a
Large ribosomal subunit protein bL36 Chain: b
Small ribosomal subunit protein uS2 Chain: c
Small ribosomal subunit protein uS3 Chain: d
Small ribosomal subunit protein uS4 Chain: e
Small ribosomal subunit protein uS5 Chain: f
Small ribosomal subunit protein bS6 Chain: g
Small ribosomal subunit protein uS7 Chain: h
Small ribosomal subunit protein uS8 Chain: i
Small ribosomal subunit protein uS9 Chain: j
Small ribosomal subunit protein uS10 Chain: k
Small ribosomal subunit protein uS11 Chain: l
Small ribosomal subunit protein uS12 Chain: m
Small ribosomal subunit protein uS13 Chain: n
Small ribosomal subunit protein uS14 Chain: o
Small ribosomal subunit protein uS15 Chain: p
Small ribosomal subunit protein bS16 Chain: q
Small ribosomal subunit protein uS17 Chain: r
Small ribosomal subunit protein bS18 Chain: s
Small ribosomal subunit protein uS19 Chain: t
Small ribosomal subunit protein bS20 Chain: u
Small ribosomal subunit protein bTHX Chain: v
23S ribosomal RNA Chain: w
5S ribosomal RNA Chain: x
16S ribosomal RNA Chain: y
P-site tRNAfMET Chain: z
E-site tRNA Chain: 2
Messenger RNA Chain: 3

Ligands and Environments

No bound ligands

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALS BEAMLINE 12.3.1
Spacegroup: I422
Unit cell:
a: 507.21Å b: 507.21Å c: 692.51Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.327 0.327 0.351