› 4v5o

X-ray diffraction
3.93Å resolution

CRYSTAL STRUCTURE OF THE EUKARYOTIC 40S RIBOSOMAL SUBUNIT IN COMPLEX WITH INITIATION FACTOR 1.

Released:
DOI: 10.2210/pdb4v5o/pdb
Model geometry
Fit model/data
PDB entry 4v5o coloured by chain, front view.
PDB entry 4v5o coloured by chain, side view.
PDB entry 4v5o coloured by chain, top view.
Source organism: Tetrahymena thermophila
Primary publication:
Crystal structure of the eukaryotic 40S ribosomal subunit in complex with initiation factor 1.
Rabl J, Leibundgut M, Ataide SF, Haag A, Ban N
Science 331 730-6 (2011)
PMID: 21205638

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 35-mer (preferred)
PDBe Complex ID:
PDB-CPX-107444 (preferred)
Entry contents:
34 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (35 distinct):
40S ribosomal protein S28e, putative Chains: A1, B1
Molecule details ›
Chains: A1, B1
Length: 68 amino acids
Theoretical weight: 7.63 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: Q234G5 (Residues: 1-68; Coverage: 100%)
Gene name: TTHERM_00105330
Sequence domains: Ribosomal protein S28e
40S ribosomal protein S8 Chains: A2, B2
Molecule details ›
Chains: A2, B2
Length: 208 amino acids
Theoretical weight: 24.25 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: Q22AV0 (Residues: 1-208; Coverage: 100%)
Gene name: TTHERM_01151600
Sequence domains: Ribosomal protein S8e
RPS7E Chains: A3, B3
Small ribosomal subunit protein eS1 Chains: A4, B4
40S ribosomal protein S26 Chains: A5, B5
40S ribosomal protein S27 Chains: A6, B6
Plectin/eS10 N-terminal domain-containing protein Chains: A7, B7
RPS25E Chains: A8, B8
RPS31E Chains: A9, B9
RPS0E Chains: AB, BB
40S ribosomal protein S3 Chains: AC, BC
Small ribosomal subunit protein uS4 N-terminal domain-containing protein Chains: AD, BD
Small ribosomal subunit protein uS5 Chains: AE, BE
EIF1 Chains: AF, BF
Small ribosomal subunit protein uS7 domain-containing protein Chains: AG, BG
40S ribosomal protein S15a Chains: AH, BH
RPS16E, 40S RIBOSOMAL PROTEIN RPS16E Chains: AI, BI
Small ribosomal subunit protein uS10 domain-containing protein Chains: AJ, BJ
RPS14E Chains: AK, BK
Small ribosomal subunit protein uS12 Chains: AL, BL
RPS18E Chains: AM, BM
40S ribosomal protein S29 Chains: AN, BN
RPS13E Chains: AO, BO
40S ribosomal protein S29 Chains: AP, BP
Small ribosomal subunit protein uS17 N-terminal domain-containing protein Chains: AQ, BQ
Receptor of activated kinase C 1A, component of 40S small ribosomal subunit Chains: AR, BR
RPS15E Chains: AS, BS
RPS19E Chains: AT, BT
Ribosomal protein eL8/eL30/eS12/Gadd45 domain-containing protein Chains: AU, BU
RPS17E Chains: AV, BV
Small ribosomal subunit protein eS4 Chains: AW, BW
RPS30E Chains: AX, BX
40S ribosomal protein S6 Chains: AY, BY
RPS21E Chains: AZ, BZ
18S Ribosomal RNA Chains: AA, BA

Ligands and Environments

2 bound ligands:
Ligand MG 184 x MG
Ligand ZN 8 x ZN
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2
Unit cell:
a: 320.52Å b: 362.21Å c: 412.11Å
α: 90° β: 109.61° γ: 90°
R-values:
R R work R free
0.206 0.206 0.243
Expression system: Escherichia coli

Quick links

Citations

71 review citations

The scanning mechanism of eukaryotic translation initiation.
Hinnebusch AG. (2014)
70 more

4 mentions without citation

Interdependent action of KH domain proteins Krr1 and Dim2 drive the 40S platform assembly.
Sturm et al. (2017)
3 more