› 4v7o

X-ray diffraction
3Å resolution

Proteasome Activator Complex

Released:
DOI: 10.2210/pdb4v7o/pdb
PDB entry 4v7o coloured by chain, front view.
PDB entry 4v7o coloured by chain, side view.
PDB entry 4v7o coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structure of a Blm10 complex reveals common mechanisms for proteasome binding and gate opening.
Sadre-Bazzaz K, Whitby FG, Robinson H, Formosa T, Hill CP
Mol Cell 37 728-35 (2010)
PMID: 20227375

Function and Biology Details

Reaction catalysed: 
Cleavage of peptide bonds with very broad specificity.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 34-mer (preferred)
PDBe Complex ID:
PDB-CPX-149136 (preferred)
Entry contents:
17 distinct polypeptide molecules
Macromolecules (17 distinct):
Proteasome subunit alpha type-1 Chains: AA, AC, BA, BO
Molecule details ›
Chains: AA, AC, BA, BO
Length: 243 amino acids
Theoretical weight: 27.32 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P21243 (Residues: 10-252; Coverage: 96%)
Gene names: PRC2, PRS2, SCL1, YGL011C
Sequence domains:
Proteasome subunit alpha type-2 Chains: AG, AS, BB, BP
Molecule details ›
Chains: AG, AS, BB, BP
Length: 231 amino acids
Theoretical weight: 25.11 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P23639 (Residues: 20-250; Coverage: 92%)
Gene names: PRE8, PRS4, YML092C
Sequence domains:
Proteasome subunit alpha type-3 Chains: AH, AT, BC, BQ
Molecule details ›
Chains: AH, AT, BC, BQ
Length: 232 amino acids
Theoretical weight: 25.61 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P23638 (Residues: 14-245; Coverage: 90%)
Gene names: PRE9, PRS5, YGR135W
Sequence domains:
Proteasome subunit alpha type-4 Chains: AI, AU, BD, BR
Molecule details ›
Chains: AI, AU, BD, BR
Length: 227 amino acids
Theoretical weight: 25.38 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P40303 (Residues: 17-243; Coverage: 89%)
Gene names: PRE6, YOL038W
Sequence domains:
Proteasome subunit alpha type-5 Chains: AJ, AV, BE, BS
Molecule details ›
Chains: AJ, AV, BE, BS
Length: 250 amino acids
Theoretical weight: 27.57 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P32379 (Residues: 1-250; Coverage: 96%)
Gene names: DOA5, G9155, PUP2, YGR253C
Sequence domains:
Proteasome subunit alpha type-6 Chains: AK, AW, BF, BT
Molecule details ›
Chains: AK, AW, BF, BT
Length: 234 amino acids
Theoretical weight: 25.63 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P40302 (Residues: 1-234; Coverage: 100%)
Gene names: PRE5, YM9924.06, YMR314W
Sequence domains:
Probable proteasome subunit alpha type-7 Chains: AL, AX, BG, BU
Molecule details ›
Chains: AL, AX, BG, BU
Length: 244 amino acids
Theoretical weight: 26.89 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P21242 (Residues: 5-248; Coverage: 85%)
Gene names: O6650, PRC1, PRE10, PRS1, YOR362C
Sequence domains:
Proteasome subunit beta type-1 Chains: AB, AD, BH, BV
Molecule details ›
Chains: AB, AD, BH, BV
Length: 196 amino acids
Theoretical weight: 21.49 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P38624 (Residues: 21-215; Coverage: 91%)
Gene names: J1407, PRE3, YJL001W
Sequence domains: Proteasome subunit
Proteasome subunit beta type-2 Chains: AM, AY, BI, BW
Molecule details ›
Chains: AM, AY, BI, BW
Length: 222 amino acids
Theoretical weight: 23.99 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P25043 (Residues: 30-251; Coverage: 85%)
Gene names: PUP1, YOR157C
Sequence domains:
Proteasome subunit beta type-3 Chains: AN, AZ, BJ, BX
Molecule details ›
Chains: AN, AZ, BJ, BX
Length: 204 amino acids
Theoretical weight: 22.5 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P25451 (Residues: 2-205; Coverage: 100%)
Gene names: PUP3, YER094C
Sequence domains: Proteasome subunit
Proteasome subunit beta type-4 Chains: A1, AO, BK, BY
Molecule details ›
Chains: A1, AO, BK, BY
Length: 198 amino acids
Theoretical weight: 22.55 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P22141 (Residues: 1-198; Coverage: 100%)
Gene names: PRE1, YER012W
Sequence domains: Proteasome subunit
Proteasome subunit beta type-5 Chains: A2, AP, BL, BZ
Molecule details ›
Chains: A2, AP, BL, BZ
Length: 212 amino acids
Theoretical weight: 23.35 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P30656 (Residues: 76-287; Coverage: 74%)
Gene names: DOA3, P8283.10, PRE2, PRG1, YPR103W
Sequence domains: Proteasome subunit
Proteasome subunit beta type-6 Chains: A3, AQ, B1, BM
Molecule details ›
Chains: A3, AQ, B1, BM
Length: 222 amino acids
Theoretical weight: 24.88 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P23724 (Residues: 20-241; Coverage: 92%)
Gene names: PRE7, PRS3, PTS1, YBL0407, YBL041W
Sequence domains: Proteasome subunit
Proteasome subunit beta type-7 Chains: A4, AR, B2, BN
Molecule details ›
Chains: A4, AR, B2, BN
Length: 233 amino acids
Theoretical weight: 25.95 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P30657 (Residues: 34-266; Coverage: 88%)
Gene names: PRE4, YFR050C
Sequence domains: Proteasome subunit
Proteasome activator BLM10 Chains: AE, AF, B3, B6
Molecule details ›
Chains: AE, AF, B3, B6
Length: 76 amino acids
Theoretical weight: 9.1 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P43583 (Residues: 79-154; Coverage: 4%)
Gene names: BLM10, BLM3, YFL006W, YFL007W
Sequence domains: Proteasome-substrate-size regulator, N-terminal
Proteasome activator BLM10 Chains: A5, A7, B4, B7
Molecule details ›
Chains: A5, A7, B4, B7
Length: 799 amino acids
Theoretical weight: 92.57 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P43583 (Residues: 239-1037; Coverage: 37%)
Gene names: BLM10, BLM3, YFL006W, YFL007W
Sequence domains: Proteasome-substrate-size regulator, mid region
Proteasome activator BLM10 Chains: A6, A8, B5, B8
Molecule details ›
Chains: A6, A8, B5, B8
Length: 997 amino acids
Theoretical weight: 114.62 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P43583 (Residues: 1147-2143; Coverage: 47%)
Gene names: BLM10, BLM3, YFL006W, YFL007W
Sequence domains: Domain of unknown function (DUF3437)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21
Unit cell:
a: 236.116Å b: 127.739Å c: 532.668Å
α: 90° β: 102.85° γ: 90°
R-values:
R R work R free
0.196 0.196 0.25

Quick links

Citations

37 review citations

Structure and Function of the 26S Proteasome.
Bard et al. (2018)
36 more

6 mentions without citation

A Practical Review of Proteasome Pharmacology.
Thibaudeau et al. (2019)
5 more