› 4v8r

X-ray diffraction
3.8Å resolution

The crystal structures of the eukaryotic chaperonin CCT reveal its functional partitioning

Released:
DOI: 10.2210/pdb4v8r/pdb
PDB entry 4v8r coloured by chain, front view.
PDB entry 4v8r coloured by chain, side view.
PDB entry 4v8r coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
The crystal structures of the eukaryotic chaperonin CCT reveal its functional partitioning.
Kalisman N, Schröder GF, Levitt M
Structure 21 540-9 (2013)
PMID: 23478063

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146270 (preferred)
Entry contents:
8 distinct polypeptide molecules
Macromolecules (8 distinct):
T-complex protein 1 subunit alpha Chains: AA, Aa, BA, Ba
Molecule details ›
Chains: AA, Aa, BA, Ba
Length: 559 amino acids
Theoretical weight: 60.56 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P12612 (Residues: 1-559; Coverage: 100%)
Gene names: CCT1, TCP1, YD8142.13, YD8142B.04, YDR212W
Sequence domains: TCP-1/cpn60 chaperonin family
T-complex protein 1 subunit beta Chains: AB, Ab, BB, Bb
Molecule details ›
Chains: AB, Ab, BB, Bb
Length: 527 amino acids
Theoretical weight: 57.28 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P39076 (Residues: 1-527; Coverage: 100%)
Gene names: BIN3, CCT2, TCP2, YIL142W
Sequence domains: TCP-1/cpn60 chaperonin family
T-complex protein 1 subunit delta Chains: AD, Ad, BD, Bd
Molecule details ›
Chains: AD, Ad, BD, Bd
Length: 528 amino acids
Theoretical weight: 57.74 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P39078 (Residues: 1-528; Coverage: 100%)
Gene names: ANC2, CCT4, TCP4, YDL143W
Sequence domains: TCP-1/cpn60 chaperonin family
T-complex protein 1 subunit epsilon Chains: AE, Ae, BE, Be
Molecule details ›
Chains: AE, Ae, BE, Be
Length: 562 amino acids
Theoretical weight: 62 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P40413 (Residues: 1-562; Coverage: 100%)
Gene names: CCT5, J1752, TCP5, YJR064W
Sequence domains: TCP-1/cpn60 chaperonin family
T-complex protein 1 subunit gamma Chains: AG, Ag, BG, Bg
Molecule details ›
Chains: AG, Ag, BG, Bg
Length: 590 amino acids
Theoretical weight: 64.94 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P39077 (Residues: 1-374, 375-534; Coverage: 100%)
Gene names: BIN2, CCT3, J1336, TCP3, YJL014W
Sequence domains: TCP-1/cpn60 chaperonin family
T-complex protein 1 subunit eta Chains: AH, Ah, BH, Bh
Molecule details ›
Chains: AH, Ah, BH, Bh
Length: 550 amino acids
Theoretical weight: 59.8 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P42943 (Residues: 1-550; Coverage: 100%)
Gene names: CCT7, J0804, YJL111W
Sequence domains: TCP-1/cpn60 chaperonin family
T-complex protein 1 subunit theta Chains: AQ, Aq, BQ, Bq
Molecule details ›
Chains: AQ, Aq, BQ, Bq
Length: 568 amino acids
Theoretical weight: 61.74 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P47079 (Residues: 1-568; Coverage: 100%)
Gene names: CCT8, J1374, YJL008C
Sequence domains: TCP-1/cpn60 chaperonin family
T-complex protein 1 subunit zeta Chains: AZ, Az, BZ, Bz
Molecule details ›
Chains: AZ, Az, BZ, Bz
Length: 546 amino acids
Theoretical weight: 60 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P39079 (Residues: 1-546; Coverage: 100%)
Gene names: CCT6, TCP20, TCP6, YD9395.21, YDR188W
Sequence domains: TCP-1/cpn60 chaperonin family

Ligands and Environments

3 bound ligands:
Ligand ADP 32 x ADP
Ligand BEF 32 x BEF
Ligand MG 32 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P1
Unit cell:
a: 159.1Å b: 162.54Å c: 268.1Å
α: 85.23° β: 81.15° γ: 61.17°
R-values:
R R work R free
0.248 0.248 0.284
Expression system: Saccharomyces cerevisiae

Quick links

Citations

9 review citations

In vivo aspects of protein folding and quality control.
Balchin et al. (2016)
8 more

4 mentions without citation

Structure of the human TRiC/CCT Subunit 5 associated with hereditary sensory neuropathy.
Pereira et al. (2017)
3 more