4v8y

Electron Microscopy
4.3Å resolution

Cryo-EM reconstruction of the 80S-eIF5B-Met-itRNAMet Eukaryotic Translation Initiation Complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 87-mer (preferred)
PDBe Complex ID:
PDB-CPX-126770 (preferred)
Entry contents:
80 distinct polypeptide molecules
6 distinct RNA molecules
Macromolecules (86 distinct):
Small ribosomal subunit protein eS26A Chain: A0
Molecule details ›
Chain: A0
Length: 119 amino acids
Theoretical weight: 13.54 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P39938 (Residues: 1-119; Coverage: 100%)
Gene names: G1355, RPS26, RPS26A, YGL189C
Sequence domains: Ribosomal protein S26e
Small ribosomal subunit protein eS27A Chain: A1
Molecule details ›
Chain: A1
Length: 82 amino acids
Theoretical weight: 8.89 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P35997 (Residues: 1-82; Coverage: 100%)
Gene names: RPS27A, YKL156W
Sequence domains: Ribosomal protein S27
Small ribosomal subunit protein eS28A Chain: A2
Small ribosomal subunit protein uS14A Chain: A3
Small ribosomal subunit protein eS30A Chain: A4
Ubiquitin-ribosomal protein eS31 fusion protein Chain: A5
Small ribosomal subunit protein RACK1 Chain: A6
Suppressor protein STM1 Chain: A7
Small ribosomal subunit protein uS2A Chain: AA
Small ribosomal subunit protein eS1A Chain: AB
Small ribosomal subunit protein uS5 Chain: AC
Small ribosomal subunit protein uS3 Chain: AD
Small ribosomal subunit protein eS4A Chain: AE
Small ribosomal subunit protein uS7 Chain: AF
Small ribosomal subunit protein eS6A Chain: AG
Small ribosomal subunit protein eS7A Chain: AH
Small ribosomal subunit protein eS8A Chain: AI
Small ribosomal subunit protein uS4A Chain: AJ
Small ribosomal subunit protein eS10A Chain: AK
Small ribosomal subunit protein uS17A Chain: AL
Small ribosomal subunit protein eS12 Chain: AM
Small ribosomal subunit protein uS15 Chain: AN
Small ribosomal subunit protein uS11A Chain: AO
Small ribosomal subunit protein uS19 Chain: AP
Small ribosomal subunit protein uS9A Chain: AQ
Small ribosomal subunit protein eS17A Chain: AR
Small ribosomal subunit protein uS13A Chain: AS
Small ribosomal subunit protein eS19A Chain: AT
Small ribosomal subunit protein uS10 Chain: AU
Small ribosomal subunit protein eS21A Chain: AV
Small ribosomal subunit protein uS8A Chain: AW
Small ribosomal subunit protein uS12A Chain: AX
Small ribosomal subunit protein eS24A Chain: AY
Small ribosomal subunit protein eS25A Chain: AZ
Large ribosomal subunit protein uL2B Chain: BA
Large ribosomal subunit protein uL3 Chain: BB
Large ribosomal subunit protein uL4A Chain: BC
Large ribosomal subunit protein uL18 Chain: BD
Large ribosomal subunit protein eL6A Chain: BE
Large ribosomal subunit protein uL30A Chain: BF
Large ribosomal subunit protein eL8A Chain: BG
Large ribosomal subunit protein uL6A Chain: BH
Large ribosomal subunit protein uL16 Chain: BI
Large ribosomal subunit protein uL5A Chain: BJ
60S RIBOSOMAL PROTEIN L11-A Chain: BK
Large ribosomal subunit protein eL13A Chain: BL
Large ribosomal subunit protein eL14B Chain: BM
Large ribosomal subunit protein eL15A Chain: BN
Large ribosomal subunit protein uL13A Chain: BO
Large ribosomal subunit protein uL22A Chain: BP
Large ribosomal subunit protein eL18A Chain: BQ
Large ribosomal subunit protein eL19 Chain: BR
Large ribosomal subunit protein eL20B Chain: BS
Large ribosomal subunit protein eL21A Chain: BT
Large ribosomal subunit protein eL22A Chain: BU
Large ribosomal subunit protein uL14A Chain: BV
Large ribosomal subunit protein eL24A Chain: BW
Large ribosomal subunit protein uL23 Chain: BX
Large ribosomal subunit protein uL24A Chain: BY
Large ribosomal subunit protein eL27A Chain: BZ
Large ribosomal subunit protein uL15 Chain: Ba
Large ribosomal subunit protein eL29 Chain: Bb
Large ribosomal subunit protein eL30 Chain: Bc
Large ribosomal subunit protein eL31A Chain: Bd
Large ribosomal subunit protein eL32 Chain: Be
Large ribosomal subunit protein eL33A Chain: Bf
Large ribosomal subunit protein eL34A Chain: Bg
Large ribosomal subunit protein uL29B Chain: Bh
Large ribosomal subunit protein eL36A Chain: Bi
Large ribosomal subunit protein eL37A Chain: Bj
Large ribosomal subunit protein eL38 Chain: Bk
Large ribosomal subunit protein eL39 Chain: Bl
Ubiquitin-ribosomal protein eL40A fusion protein Chain: Bm
Small ribosomal subunit protein eS32B Chain: Bn
Large ribosomal subunit protein eL42A Chain: Bo
Large ribosomal subunit protein uL10 Chain: Bq
60S ACIDIC RIBOSOMAL PROTEIN P1 Chain: Br
60S ACIDIC RIBOSOMAL PROTEIN P2 Chain: Bs
50S RIBOSOMAL PROTEIN L1 Chains: By, CL
Eukaryotic translation initiation factor 5B Chain: CP
18S Ribosomal RNA Chain: B2
25S ribosomal RNA Chain: B5
5S ribosomal RNA Chain: B7
5.8S ribosomal RNA Chain: B8
EUKARYOTIC RIBOSOMAL L1_RRNA Chain: CN
EUKARYOTIC RIBOSOMAL P_E TRNA Chain: CW

Ligands and Environments

No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 4.3Å
Relevant EMDB volumes: EMD-2421