› 4v96

X-ray diffraction
3.8Å resolution

The structure of a 1.8 MDa viral genome injection device suggests alternative infection mechanisms

Released:
DOI: 10.2210/pdb4v96/pdb
PDB entry 4v96 coloured by chain, front view.
PDB entry 4v96 coloured by chain, side view.
PDB entry 4v96 coloured by chain, top view.
Source organism: Lactococcus phage TP901-1
Primary publication:
Structure of the phage TP901-1 1.8 MDa baseplate suggests an alternative host adhesion mechanism.
Veesler D, Spinelli S, Mahony J, Lichière J, Blangy S, Bricogne G, Legrand P, Ortiz-Lombardia M, Campanacci V, van Sinderen D, Cambillau C
Proc Natl Acad Sci U S A 109 8954-8 (2012)
PMID: 22611190

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 78-mer (preferred)
PDBe Complex ID:
PDB-CPX-189621 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
ORF48 Chains: AA, AB, AC, AD, AE, AF, AG, AH, AI, AJ, AK, AL, AM, AN, AO, AP, AQ, AR
Molecule details ›
Chains: AA, AB, AC, AD, AE, AF, AG, AH, AI, AJ, AK, AL, AM, AN, AO, AP, AQ, AR
Length: 299 amino acids
Theoretical weight: 33.88 KDa
Source organism: Lactococcus phage TP901-1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9AZ56 (Residues: 1-299; Coverage: 100%)
Sequence domains:
ORF46 Chains: AS, AT, AU, AV, AW, AX
Molecule details ›
Chains: AS, AT, AU, AV, AW, AX
Length: 253 amino acids
Theoretical weight: 29.09 KDa
Source organism: Lactococcus phage TP901-1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9AZ58 (Residues: 2-253; Coverage: 100%)
BPP Chains: B1, B2, BA, BB, BC, BD, BE, BF, BG, BH, BI, BJ, BK, BL, BM, BN, BO, BP, BQ, BR, BS, BT, BU, BV, BW, BX, BY, BZ, Ba, Bb, Bc, Bd, Be, Bf, Bg, Bh, Bi, Bj, Bk, Bl, Bm, Bn, Bo, Bp, Bq, Br, Bs, Bt, Bu, Bv, Bw, Bx, By, Bz
Molecule details ›
Chains: B1, B2, BA, BB, BC, BD, BE, BF, BG, BH, BI, BJ, BK, BL, BM, BN, BO, BP, BQ, BR, BS, BT, BU, BV, BW, BX, BY, BZ, Ba, Bb, Bc, Bd, Be, Bf, Bg, Bh, Bi, Bj, Bk, Bl, Bm, Bn, Bo, Bp, Bq, Br, Bs, Bt, Bu, Bv, Bw, Bx, By, Bz
Length: 173 amino acids
Theoretical weight: 18.37 KDa
Source organism: Lactococcus phage TP901-1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9G096 (Residues: 1-163; Coverage: 100%)
Gene name: bpp
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P21
Unit cell:
a: 163.53Å b: 287.36Å c: 323.14Å
α: 90° β: 101.73° γ: 90°
R-values:
R R work R free
0.237 0.237 0.264
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

18 review citations

Molecular architecture of tailed double-stranded DNA phages.
Fokine et al. (2014)
17 more

8 mentions without citation

Molecular Basis of Bacterial Host Interactions by Gram-Positive Targeting Bacteriophages.
Dunne et al. (2018)
7 more