› 4w4h

X-ray diffraction
2.89Å resolution

Escherichia coli tryptophanase in holo form

Released:
DOI: 10.2210/pdb4w4h/pdb
PDB entry 4w4h coloured by chain, front view.
PDB entry 4w4h coloured by chain, side view.
PDB entry 4w4h coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structures of Escherichia coli tryptophanase in holo and 'semi-holo' forms.
Kogan A, Raznov L, Gdalevsky GY, Cohen-Luria R, Almog O, Parola AH, Goldgur Y
Acta Crystallogr F Struct Biol Commun 71 286-90 (2015)
PMID: 25760702

Function and Biology Details

Reaction catalysed: 
(1a) L-tryptophan = indole + 2-aminoprop-2-enoate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141663 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tryptophanase Chains: A, B
Molecule details ›
Chains: A, B
Length: 467 amino acids
Theoretical weight: 52.54 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A853 (Residues: 5-471; Coverage: 99%)
Gene names: JW3686, b3708, ind, tnaA
Sequence domains: Beta-eliminating lyase
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand LLP 2 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P43212
Unit cell:
a: 112.44Å b: 112.44Å c: 232.65Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.227 0.278
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

The response of Escherichia coli biofilm to salicylic acid.
Cattò et al. (2017)
3 more

1 mention without citation

Structures of Escherichia coli tryptophanase in holo and 'semi-holo' forms.
Kogan et al. (2015)