› 4w68

X-ray diffraction
2Å resolution

Cytoplasmically Produced Homodimeric Single Domain Antibody (sdAb) C22A/C99V variant against Staphylococcal enterotoxin B (SEB)

Released:
DOI: 10.2210/pdb4w68/pdb
PDB entry 4w68 coloured by chain, front view.
PDB entry 4w68 coloured by chain, side view.
PDB entry 4w68 coloured by chain, top view.
Source organism: Lama glama
Primary publication:
Structural and mutational analysis of a monomeric and dimeric form of a single domain antibody with implications for protein misfolding.
George J, Compton JR, Leary DH, Olson MA, Legler PM
Proteins 82 3101-16 (2014)
PMID: 25136772

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-230418 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Single domain antibody Chains: A, B
Molecule details ›
Chains: A, B
Length: 133 amino acids
Theoretical weight: 14.35 KDa
Source organism: Lama glama
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR-H
Spacegroup: C2221
Unit cell:
a: 66.893Å b: 72.044Å c: 116.103Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.199 0.242
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Enhancing Stability of Camelid and Shark Single Domain Antibodies: An Overview.
Goldman et al. (2017)
10 other articles

1 mention without citation

Sequence Tolerance of a Single-Domain Antibody with a High Thermal Stability: Comparison of Computational and Experimental Fitness Profiles.
Olson et al. (2019)