› 4w74

X-ray diffraction
2.1Å resolution

Crystal Structure of Full-Length Split GFP Mutant E115C/T118H With Metal Mediated Crystal Contacts, P 1 21 1 Space Group

Released:
DOI: 10.2210/pdb4w74/pdb
PDB entry 4w74 coloured by chain, front view.
PDB entry 4w74 coloured by chain, side view.
PDB entry 4w74 coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
A Suite of Engineered GFP Molecules for Oligomeric Scaffolding.
Leibly DJ, Arbing MA, Pashkov I, DeVore N, Waldo GS, Terwilliger TC, Yeates TO
Structure 23 1754-1768 (2015)
PMID: 26278175

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165301 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
fluorescent protein E115C/T118H Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 229 amino acids
Theoretical weight: 26.07 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand ZN 6 x ZN
1 modified residue:
Ligand CRO 8 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 67.44Å b: 119.79Å c: 130.57Å
α: 90° β: 89.99° γ: 90°
R-values:
R R work R free
0.214 0.212 0.235
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Split Green Fluorescent Proteins: Scope, Limitations, and Outlook.
Romei et al. (2019)
3 more

1 mention without citation

A Suite of Engineered GFP Molecules for Oligomeric Scaffolding.
Leibly et al. (2015)