› 4w78

X-ray diffraction
1.54Å resolution

Crystal structure of the ChsH1-ChsH2 complex from Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb4w78/pdb
PDB entry 4w78 coloured by chain, front view.
PDB entry 4w78 coloured by chain, side view.
PDB entry 4w78 coloured by chain, top view.
Source organisms:
Primary publication:
A distinct MaoC-like enoyl-CoA hydratase architecture mediates cholesterol catabolism in Mycobacterium tuberculosis.
Yang M, Guja KE, Thomas ST, Garcia-Diaz M, Sampson NS
ACS Chem Biol 9 2632-45 (2014)
PMID: 25203216

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
hetero octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-165308 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Hydratase ChsH1 Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 178 amino acids
Theoretical weight: 19.16 KDa
Source organism: Mycobacterium tuberculosis CCDC5180
Expression system: Escherichia coli
Structure domains: Hotdog Thioesterase
Hydratase ChsH2 Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 127 amino acids
Theoretical weight: 13.76 KDa
Source organism: Mycobacterium tuberculosis SUMu008
Expression system: Escherichia coli
Structure domains: Hotdog Thioesterase

Ligands and Environments

4 bound ligands:
Ligand CD 13 x CD
Ligand CL 4 x CL
Ligand NA 1 x NA
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21
Unit cell:
a: 51.785Å b: 134.096Å c: 90.42Å
α: 90° β: 91.58° γ: 90°
R-values:
R R work R free
0.177 0.177 0.205
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Cholesterol and fatty acids grease the wheels of Mycobacterium tuberculosis pathogenesis.
Wilburn et al. (2018)
3 more

2 mentions without citation

A distinct MaoC-like enoyl-CoA hydratase architecture mediates cholesterol catabolism in Mycobacterium tuberculosis.
Yang et al. (2014)
1 more