› 4w85

X-ray diffraction
1.92Å resolution

Crystal structure of XEG5A, a GH5 xyloglucan-specific endo-beta-1,4-glucanase from ruminal metagenomic library, in complex with glucose

Released:
DOI: 10.2210/pdb4w85/pdb
PDB entry 4w85 coloured by chain, front view.
PDB entry 4w85 coloured by chain, side view.
PDB entry 4w85 coloured by chain, top view.
Source organism: uncultured bacterium
Primary publication:
Structural basis for xyloglucan specificity and α-d-Xylp(1 → 6)-D-Glcp recognition at the -1 subsite within the GH5 family.
Dos Santos CR, Cordeiro RL, Wong DW, Murakami MT
Biochemistry 54 1930-42 (2015)
PMID: 25714929

Function and Biology Details

Reaction catalysed: 
Xyloglucan + H(2)O = xyloglucan oligosaccharides
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112370 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycoside hydrolase family 5 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 339 amino acids
Theoretical weight: 38.12 KDa
Source organism: uncultured bacterium
Expression system: Escherichia coli
UniProt:
  • Canonical: D2K7Z0 (Residues: 92-430; Coverage: 83%)
Sequence domains: Cellulase (glycosyl hydrolase family 5)
Structure domains: Glycosidases

Ligands and Environments

2 bound ligands:
Ligand BGC 2 x BGC
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE W01B-MX2
Spacegroup: P31
Unit cell:
a: 97.604Å b: 97.604Å c: 96.241Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.181 0.179 0.216
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Recent structural insights into the enzymology of the ubiquitous plant cell wall glycan xyloglucan.
Attia et al. (2016)
1 more

1 mention without citation

Metadata Analysis Approaches for Understanding and Improving the Functional Involvement of Rumen Microbial Consortium in Digestion and Metabolism of Plant Biomass.
Kameshwar et al. (2019)