4w8j

X-ray diffraction
2.78Å resolution

Structure of the full-length insecticidal protein Cry1Ac reveals intriguing details of toxin packaging into in vivo formed crystals

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-138407 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pesticidal crystal protein Cry1Ac Chain: A
Molecule details ›
Chain: A
Length: 1184 amino acids
Theoretical weight: 133.98 KDa
Source organism: Bacillus thuringiensis serovar kurstaki str. HD73
Expression system: Bacillus thuringiensis
UniProt:
  • Canonical: P05068 (Residues: 1-1178; Coverage: 100%)
Gene names: cry1A(c), cry1Ac, cry218, cryIA(c)
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P41212
Unit cell:
a: 87.314Å b: 87.314Å c: 266.396Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.226 0.332
Expression system: Bacillus thuringiensis