› 4w8k

X-ray diffraction
2.13Å resolution

Crystal structure of a putative Cas1 enzyme from Vibrio phage ICP1

Released:
DOI: 10.2210/pdb4w8k/pdb
PDB entry 4w8k coloured by chain, front view.
PDB entry 4w8k coloured by chain, side view.
PDB entry 4w8k coloured by chain, top view.
Source organism: Vibrio phage ICP1_2005_A
Entry authors: Stogios PJ, Wawrzak Z, Onopriyeno O, Yim V, Savchenko A, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123293 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cas1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 323 amino acids
Theoretical weight: 36.72 KDa
Source organism: Vibrio phage ICP1_2005_A
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: F1D4K1 (Residues: 1-302; Coverage: 100%)
Gene name: TUST1-15_00465
Sequence domains: CRISPR associated protein Cas1
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand K 1 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P212121
Unit cell:
a: 70.59Å b: 83.32Å c: 133.98Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.174 0.207
Expression system: Escherichia coli BL21(DE3)

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