› 4w8o

X-ray diffraction
2.05Å resolution

Structure of the luciferase-like enzyme from the nonluminescent Zophobas morio mealworm

Released:
DOI: 10.2210/pdb4w8o/pdb
PDB entry 4w8o coloured by chain, front view.
PDB entry 4w8o coloured by chain, side view.
PDB entry 4w8o coloured by chain, top view.
Source organism: Zophobas atratus
Entry authors: Santos CR, Prado RA, Viviani V, Murakami MT

Function and Biology Details

Reaction catalysed: 
D-firefly luciferin + O(2) + ATP = firefly oxyluciferin + CO(2) + AMP + diphosphate + light
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102626 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Luciferin 4-monooxygenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 427 amino acids
Theoretical weight: 47.92 KDa
Source organism: Zophobas atratus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0R4I967 (Residues: 1-427; Coverage: 100%)
Sequence domains: AMP-binding enzyme
Structure domains: N-terminal domain of ligase-like

Ligands and Environments

2 bound ligands:
Ligand P33 2 x P33
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE W01B-MX2
Spacegroup: P212121
Unit cell:
a: 87.774Å b: 91.005Å c: 110.294Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.188 0.232
Expression system: Escherichia coli

Quick links