› 4wba

X-ray diffraction
1.8Å resolution

Q/E mutant SA11 NSP4_CCD

Released:
DOI: 10.2210/pdb4wba/pdb
PDB entry 4wba coloured by chain, front view.
PDB entry 4wba coloured by chain, side view.
PDB entry 4wba coloured by chain, top view.
Source organism: Simian rotavirus A/SA11
Primary publication:
Structural plasticity of the coiled-coil domain of rotavirus NSP4.
Sastri NP, Viskovska M, Hyser JM, Tanner MR, Horton LB, Sankaran B, Prasad BV, Estes MK
J Virol 88 13602-12 (2014)
PMID: 25231315

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131650 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Non-structural glycoprotein 4 Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 52 amino acids
Theoretical weight: 6.22 KDa
Source organism: Simian rotavirus A/SA11
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O92323 (Residues: 95-146; Coverage: 30%)
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

2 bound ligands:
Ligand GOL 2 x GOL
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P21
Unit cell:
a: 39.857Å b: 53.469Å c: 52.543Å
α: 90° β: 95.84° γ: 90°
R-values:
R R work R free
0.19 0.187 0.237
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Viroporins: structure, function and potential as antiviral targets.
Scott et al. (2015)
4 more

2 mentions without citation

Structural plasticity of the coiled-coil domain of rotavirus NSP4.
Sastri et al. (2014)
1 more