› 4wc0

X-ray diffraction
3.1Å resolution

tRNA-processing enzyme with ATP

Released:
DOI: 10.2210/pdb4wc0/pdb
Model geometry
Fit model/data
PDB entry 4wc0 coloured by chain, front view.
PDB entry 4wc0 coloured by chain, side view.
PDB entry 4wc0 coloured by chain, top view.
Source organism: Aquifex aeolicus VF5
Primary publication:
Measurement of Acceptor-TΨC Helix Length of tRNA for Terminal A76-Addition by A-Adding Enzyme.
Yamashita S, Martinez A, Tomita K
Structure 23 830-842 (2015)
PMID: 25914059

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130370 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
A-adding tRNA nucleotidyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 396 amino acids
Theoretical weight: 46.55 KDa
Source organism: Aquifex aeolicus VF5
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O66728 (Residues: 443-824; Coverage: 46%)
Gene name: aq_411
Sequence domains: Poly A polymerase head domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ATP 2 x ATP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P21
Unit cell:
a: 57.71Å b: 109.06Å c: 85.09Å
α: 90° β: 109.84° γ: 90°
R-values:
R R work R free
0.215 0.213 0.263
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

The tRNA Elbow in Structure, Recognition and Evolution.
Zhang et al. (2016)
3 more