› 4wcj

X-ray diffraction
1.7Å resolution

Structure of IcaB from Ammonifex degensii

Released:
DOI: 10.2210/pdb4wcj/pdb
PDB entry 4wcj coloured by chain, front view.
PDB entry 4wcj coloured by chain, side view.
PDB entry 4wcj coloured by chain, top view.
Source organism: Ammonifex degensii KC4
Primary publication:
Structural basis for the De-N-acetylation of Poly-β-1,6-N-acetyl-D-glucosamine in Gram-positive bacteria.
Little DJ, Bamford NC, Pokrovskaya V, Robinson H, Nitz M, Howell PL
J Biol Chem 289 35907-17 (2014)
PMID: 25359777

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111876 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NodB homology domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 264 amino acids
Theoretical weight: 29.77 KDa
Source organism: Ammonifex degensii KC4
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: C9RCK9 (Residues: 23-280; Coverage: 87%)
Gene name: Adeg_0846
Sequence domains: Polysaccharide deacetylase
Structure domains: Glycoside hydrolase/deacetylase

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: I4
Unit cell:
a: 84.686Å b: 84.686Å c: 71.94Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.204 0.231
Expression system: Escherichia coli BL21

Quick links

Citations

4 review citations

Insights into the structure and function of membrane-integrated processive glycosyltransferases.
Bi et al. (2015)
3 more

3 mentions without citation

Enzymatic modifications of exopolysaccharides enhance bacterial persistence.
Whitfield et al. (2015)
2 more