› 4wd8

X-ray diffraction
2.3Å resolution

Crystal structure of a bacterial Bestrophin homolog from Klebsiella pneumoniae

Released:
DOI: 10.2210/pdb4wd8/pdb
PDB entry 4wd8 coloured by chain, front view.
PDB entry 4wd8 coloured by chain, side view.
PDB entry 4wd8 coloured by chain, top view.
Source organism: Klebsiella pneumoniae UHKPC96
Primary publication:
Structure and selectivity in bestrophin ion channels.
Yang T, Liu Q, Kloss B, Bruni R, Kalathur RC, Guo Y, Kloppmann E, Rost B, Colecraft HM, Hendrickson WA
Science 346 355-9 (2014)
PMID: 25324390

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165322 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bestrophin domain protein Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 297 amino acids
Theoretical weight: 33.83 KDa
Source organism: Klebsiella pneumoniae UHKPC96
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand ZN 15 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P212121
Unit cell:
a: 114.079Å b: 160.034Å c: 161.881Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.196 0.214
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Bestrophin 1 and retinal disease.
Johnson et al. (2017)
12 more

6 mentions without citation

Large-scale determination of previously unsolved protein structures using evolutionary information.
Ovchinnikov et al. (2015)
5 more