› 4wdr

X-ray diffraction
2.5Å resolution

Crystal structure of haloalkane dehalogenase LinB 140A+143L+177W+211L mutant (LinB86) from Sphingobium japonicum UT26

Released:
DOI: 10.2210/pdb4wdr/pdb
PDB entry 4wdr coloured by chain, front view.
PDB entry 4wdr coloured by chain, side view.
PDB entry 4wdr coloured by chain, top view.
Source organism: Sphingobium japonicum UT26S
Primary publication:
Crystal structure of haloalkane dehalogenase LinB mutant (L177W) from Sphingobium japonicum UT26
Degtjarik O, Rezacova P, Chaloupkova R, Damborsky J, Kuta-Smatanova I
ACS Catalysis (2016)

Function and Biology Details

Reaction catalysed: 
1-haloalkane + H(2)O = a primary alcohol + halide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112655 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Haloalkane dehalogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 293 amino acids
Theoretical weight: 32.74 KDa
Source organism: Sphingobium japonicum UT26S
Expression system: Escherichia coli
UniProt:
  • Canonical: D4Z2G1 (Residues: 4-296; Coverage: 99%)
Gene names: SJA_C1-19590, linB
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

2 bound ligands:
Ligand CL 2 x CL
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: R32
Unit cell:
a: 125.937Å b: 125.937Å c: 213.818Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.167 0.165 0.196
Expression system: Escherichia coli

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