› 4we4

X-ray diffraction
2.35Å resolution

The crystal structure of hemagglutinin from 1968 H3N2 influenza virus

Released:
DOI: 10.2210/pdb4we4/pdb
PDB entry 4we4 coloured by chain, front view.
PDB entry 4we4 coloured by chain, side view.
PDB entry 4we4 coloured by chain, top view.
Source organism: Influenza A virus (A/Hong Kong/1/1968(H3N2))
Primary publication:
Structure and receptor binding preferences of recombinant human A(H3N2) virus hemagglutinins.
Yang H, Carney PJ, Chang JC, Guo Z, Villanueva JM, Stevens J
Virology 477 18-31 (2015)
PMID: 25617824

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187534 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (6 distinct):
Hemagglutinin HA1 chain Chain: A
Molecule details ›
Chain: A
Length: 321 amino acids
Theoretical weight: 35.34 KDa
Source organism: Influenza A virus (A/Hong Kong/1/1968(H3N2))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q91MA7 (Residues: 25-345; Coverage: 58%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains: Hemagglutinin (Ha1 Chain); Chain: A; domain 1
Hemagglutinin HA2 chain Chain: B
Molecule details ›
Chain: B
Length: 172 amino acids
Theoretical weight: 19.91 KDa
Source organism: Influenza A virus (A/Hong Kong/1/1968(H3N2))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q91MA7 (Residues: 346-517; Coverage: 31%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains: Hemagglutinin Ectodomain; Chain B

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
3 bound ligands:
Ligand NAG 2 x NAG
Ligand PEG 7 x PEG
Ligand PE5 1 x PE5
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: I213
Unit cell:
a: 155.506Å b: 155.506Å c: 155.506Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.193 0.236
Expression system: Trichoplusia ni

Quick links

Citations

11 review citations

Influenza Hemagglutinin and Neuraminidase: Yin⁻Yang Proteins Coevolving to Thwart Immunity.
Kosik et al. (2019)
10 more

11 mentions without citation

Design of protein-binding proteins from the target structure alone.
Cao et al. (2022)
10 more