› 4wfi

X-ray diffraction
1.45Å resolution

Crystal structure of PET-degrading cutinase Cut190 S226P mutant in Ca(2+)-free state

Released:
DOI: 10.2210/pdb4wfi/pdb
PDB entry 4wfi coloured by chain, front view.
PDB entry 4wfi coloured by chain, side view.
PDB entry 4wfi coloured by chain, top view.
Source organism: Saccharomonospora viridis
Primary publication:
Structural basis for the Ca(2+)-enhanced thermostability and activity of PET-degrading cutinase-like enzyme from Saccharomonospora viridis AHK190.
Miyakawa T, Mizushima H, Ohtsuka J, Oda M, Kawai F, Tanokura M
Appl Microbiol Biotechnol 99 4297-307 (2015)
PMID: 25492421

Function and Biology Details

Reaction catalysed: 
Cutin + H(2)O = cutin monomers
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197715 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cutinase Chain: A
Molecule details ›
Chain: A
Length: 273 amino acids
Theoretical weight: 30.29 KDa
Source organism: Saccharomonospora viridis
Expression system: Escherichia coli
UniProt:
  • Canonical: W0TJ64 (Residues: 47-304; Coverage: 85%)
Gene names: Cut190, MINT15_00360
Sequence domains: Platelet-activating factor acetylhydrolase, isoform II
Structure domains: alpha/beta hydrolase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P212121
Unit cell:
a: 57.447Å b: 65.142Å c: 69.575Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.176 0.191
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Microbial Polyethylene Terephthalate Hydrolases: Current and Future Perspectives.
Carr et al. (2020)
8 more

10 mentions without citation

Current knowledge on enzymatic PET degradation and its possible application to waste stream management and other fields.
Kawai et al. (2019)
9 more