› 4wfo

X-ray diffraction
1.14Å resolution

manganese-substituted soybean lipoxygenase-1

Released:
DOI: 10.2210/pdb4wfo/pdb
PDB entry 4wfo coloured by chain, front view.
PDB entry 4wfo coloured by chain, side view.
PDB entry 4wfo coloured by chain, top view.
Source organism: Glycine max
Entry authors: Carr CAM, Scouras AD, Klinman JP

Function and Biology Details

Reaction catalysed: 
Alpha-linolenate + O(2) = (9Z,11E,13S,15Z)-13-hydroperoxyoctadeca-9,11,15-trienoate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139953 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Seed linoleate 13S-lipoxygenase-1 Chain: A
Molecule details ›
Chain: A
Length: 858 amino acids
Theoretical weight: 96.78 KDa
Source organism: Glycine max
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P08170 (Residues: 1-839; Coverage: 100%)
Gene names: LOX1, LOX1.1
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MN 1 x MN
Ligand EDO 8 x EDO
Ligand ACT 6 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P21
Unit cell:
a: 48.123Å b: 91.497Å c: 90.897Å
α: 90° β: 93.88° γ: 90°
R-values:
R R work R free
0.108 0.108 0.128
Expression system: Escherichia coli BL21(DE3)

Quick links