› 4whb

X-ray diffraction
2.96Å resolution

Crystal structure of phenylurea hydrolase B

Released:
DOI: 10.2210/pdb4whb/pdb
PDB entry 4whb coloured by chain, front view.
PDB entry 4whb coloured by chain, side view.
PDB entry 4whb coloured by chain, top view.
Source organism: Mycolicibacterium brisbanense
Primary publication:
Evolutionary expansion of the amidohydrolase superfamily in bacteria in response to the synthetic compounds molinate and diuron.
Sugrue E, Fraser NJ, Hopkins DH, Carr PD, Khurana JL, Oakeshott JG, Scott C, Jackson CJ
Appl Environ Microbiol 81 2612-24 (2015)
PMID: 25636851

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110760 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Amidohydrolase-related domain-containing protein Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 461 amino acids
Theoretical weight: 49.75 KDa
Source organism: Mycolicibacterium brisbanense
Expression system: Mycolicibacterium smegmatis
UniProt:
  • Canonical: B8R4K0 (Residues: 1-461; Coverage: 100%)
Gene name: puhB
Sequence domains: Amidohydrolase family
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P21
Unit cell:
a: 77.374Å b: 100.526Å c: 238.551Å
α: 90° β: 98.37° γ: 90°
R-values:
R R work R free
0.255 0.252 0.309
Expression system: Mycolicibacterium smegmatis

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Citations

1 review citation

Diversity in protein domain superfamilies.
Das et al. (2015)
6 other articles

1 mention without citation

Evolutionary expansion of the amidohydrolase superfamily in bacteria in response to the synthetic compounds molinate and diuron.
Sugrue et al. (2015)