› 4wia

X-ray diffraction
2.2Å resolution

Crystal structure of flagellar accessory protein FlaH from Methanocaldococcus jannaschii

Released:
DOI: 10.2210/pdb4wia/pdb
PDB entry 4wia coloured by chain, front view.
PDB entry 4wia coloured by chain, side view.
PDB entry 4wia coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii DSM 2661
Primary publication:
Crystal structure of the flagellar accessory protein FlaH of Methanocaldococcus jannaschii suggests a regulatory role in archaeal flagellum assembly.
Meshcheryakov VA, Wolf M
Protein Sci 25 1147-55 (2016)
PMID: 27060465

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176602 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative flagella-related protein H Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 233 amino acids
Theoretical weight: 26.37 KDa
Source organism: Methanocaldococcus jannaschii DSM 2661
Expression system: Escherichia coli
UniProt:
  • Canonical: Q58309 (Residues: 1-233; Coverage: 100%)
Gene names: MJ0899, flaH
Sequence domains: KaiC
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand SO4 13 x SO4
Ligand GOL 1 x GOL
1 modified residue:
Ligand MSE 21 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P3121
Unit cell:
a: 130.96Å b: 130.96Å c: 89.081Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.207 0.206 0.233
Expression system: Escherichia coli

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Citations

4 review citations

Versatile cell surface structures of archaea.
Chaudhury et al. (2018)
3 more

2 mentions without citation

Crystal structure of the flagellar accessory protein FlaH of Methanocaldococcus jannaschii suggests a regulatory role in archaeal flagellum assembly.
Meshcheryakov et al. (2016)
1 more