› 4wj0

X-ray diffraction
2.85Å resolution

Structure of PH1245, a cas1 from Pyrococcus horikoshii

Released:
DOI: 10.2210/pdb4wj0/pdb
PDB entry 4wj0 coloured by chain, front view.
PDB entry 4wj0 coloured by chain, side view.
PDB entry 4wj0 coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Entry authors: Petit P, Brown G, Savchenko A, Yakunin AF

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129786 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CRISPR-associated endonuclease Cas1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 322 amino acids
Theoretical weight: 37.82 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O58938 (Residues: 1-322; Coverage: 100%)
Gene names: PH1245, cas1
Sequence domains: CRISPR associated protein Cas1
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 125.89Å b: 58.672Å c: 90.147Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.227 0.282
Expression system: Escherichia coli BL21(DE3)

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