4wjv

X-ray diffraction
3.2Å resolution

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
PDBe Complex ID:
PDB-CPX-219348 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Ribosome assembly protein 4 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 381 amino acids
Theoretical weight: 42.46 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P25382 (Residues: 137-515; Coverage: 74%)
Gene names: RSA4, YCR072C, YCR72C
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Maltose/maltodextrin-binding periplasmic protein Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 381 amino acids
Theoretical weight: 41.65 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEX9 (Residues: 27-393; Coverage: 99%)
Gene names: JW3994, b4034, malE
Sequence domains: Bacterial extracellular solute-binding protein
Ribosome biogenesis protein NSA2 Chains: I, J, K, L
Molecule details ›
Chains: I, J, K, L
Length: 22 amino acids
Theoretical weight: 2.48 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P40078 (Residues: 80-101; Coverage: 8%)
Gene names: NSA2, SYGP-ORF47, YER126C

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: C2
Unit cell:
a: 198.578Å b: 96.493Å c: 196.445Å
α: 90° β: 115.45° γ: 90°
R-values:
R R work R free
0.195 0.192 0.244
Expression system: Escherichia coli