› 4wko

X-ray diffraction
1.9Å resolution

Crystal structure of Helicobacter pylori 5'-methylthioadenosine/S-adenosyl homocysteine nucleosidase (MTAN) complexed with hydroxybutylthio-DADMe-Immucillin-A

Released:
DOI: 10.2210/pdb4wko/pdb
PDB entry 4wko coloured by chain, front view.
PDB entry 4wko coloured by chain, side view.
PDB entry 4wko coloured by chain, top view.
Source organism: Helicobacter pylori J99
Primary publication:
New Antibiotic Candidates against Helicobacter pylori.
Wang S, Cameron SA, Clinch K, Evans GB, Wu Z, Schramm VL, Tyler PC
J Am Chem Soc 137 14275-80 (2015)
PMID: 26494017

Function and Biology Details

Reactions catalysed: 
6-amino-6-deoxyfutalosine + H(2)O = dehypoxanthine futalosine + adenine
5'-deoxyadenosine + H(2)O = 5-deoxy-D-ribose + adenine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195684 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminodeoxyfutalosine nucleosidase Chain: A
Molecule details ›
Chain: A
Length: 245 amino acids
Theoretical weight: 26.89 KDa
Source organism: Helicobacter pylori J99
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9ZMY2 (Residues: 2-230; Coverage: 100%)
Gene names: jhp_0082, mtn, mtnN
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

1 bound ligand:
Ligand GMD 1 x GMD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: I432
Unit cell:
a: 157.256Å b: 157.256Å c: 157.256Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.158 0.19
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

Helicobacter pylori treatment in the post-antibiotics era-searching for new drug targets.
Roszczenko-Jasińska et al. (2020)
3 more

2 mentions without citation

Transition-State Analogues of Campylobacter jejuni 5'-Methylthioadenosine Nucleosidase.
Ducati et al. (2018)
1 more