› 4wlh

X-ray diffraction
1.28Å resolution

High resolution crystal structure of human kynurenine aminotransferase-I bound to PLP cofactor

Released:
DOI: 10.2210/pdb4wlh/pdb
PDB entry 4wlh coloured by chain, front view.
PDB entry 4wlh coloured by chain, side view.
PDB entry 4wlh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
High resolution crystal structures of human kynurenine aminotransferase-I bound to PLP cofactor, and in complex with aminooxyacetate.
Nadvi NA, Salam NK, Park J, Akladios FN, Kapoor V, Collyer CA, Gorrell MD, Church WB
Protein Sci 26 727-736 (2017)
PMID: 28097769

Function and Biology Details

Reactions catalysed: 
L-glutamine + phenylpyruvate = 2-oxoglutaramate + L-phenylalanine
(1a) L-kynurenine + 2-oxoglutarate = 4-(2-aminophenyl)-2,4-dioxobutanoate + L-glutamate
(1a) an L-cysteine-S-conjugate = a thiol + 2-aminoporp-2-enoate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172574 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kynurenine--oxoglutarate transaminase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 422 amino acids
Theoretical weight: 48.16 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q16773 (Residues: 1-422; Coverage: 100%)
Gene names: CCBL1, KYAT1
Sequence domains: Aminotransferase class I and II
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand LLP 2 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: C2
Unit cell:
a: 102.44Å b: 107.56Å c: 81.53Å
α: 90° β: 112.64° γ: 90°
R-values:
R R work R free
0.172 0.172 0.187
Expression system: Spodoptera frugiperda

Quick links

Citations

3 review citations

Cystathionine-β-Synthase: Molecular Regulation and Pharmacological Inhibition.
Zuhra et al. (2020)
2 more

1 mention without citation

High resolution crystal structures of human kynurenine aminotransferase-I bound to PLP cofactor, and in complex with aminooxyacetate.
Nadvi et al. (2017)