› 4wls

X-ray diffraction
2.1Å resolution

Crystal structure of the metal-free (repressor) form of E. Coli CUER, a copper efflux regulator, bound to COPA promoter DNA

Released:
DOI: 10.2210/pdb4wls/pdb
PDB entry 4wls coloured by chain, front view.
PDB entry 4wls coloured by chain, side view.
PDB entry 4wls coloured by chain, top view.
Source organisms:
Primary publication:
TRANSCRIPTION. Allosteric transcriptional regulation via changes in the overall topology of the core promoter.
Philips SJ, Canalizo-Hernandez M, Yildirim I, Schatz GC, Mondragón A, O'Halloran TV
Science 349 877-81 (2015)
PMID: 26293965

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118226 (preferred)
Entry contents:
1 distinct polypeptide molecule
4 distinct DNA molecules
Macromolecules (5 distinct):
HTH-type transcriptional regulator CueR Chains: A, B
Molecule details ›
Chains: A, B
Length: 128 amino acids
Theoretical weight: 14.6 KDa
Source organism: Escherichia coli DH5[alpha]
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A9G4 (Residues: 1-128; Coverage: 95%)
Gene names: JW0476, b0487, copR, cueR, ybbI
Sequence domains:
Structure domains: Multidrug-efflux Transporter Regulator; Chain: A; Domain 2
COPA PROMOTER DNA NON-TEMPLATE STRAND Chain: X
Molecule details ›
Chain: X
Length: 26 nucleotides
Theoretical weight: 7.95 KDa
Source organism: synthetic construct
Expression system: Not provided
COPA PROMOTER DNA TEMPLATE STRAND Chain: Y
Molecule details ›
Chain: Y
Length: 26 nucleotides
Theoretical weight: 8.03 KDa
Source organism: synthetic construct
Expression system: Not provided
COPA PROMOTER DNA TEMPLATE STRAND (ALTERNATE CONFORMATION) Chain: U
Molecule details ›
Chain: U
Length: 26 nucleotides
Theoretical weight: 8.04 KDa
Source organism: synthetic construct
Expression system: Not provided
COPA PROMOTER DNA NON-TEMPLATE STRAND (ALTERNATE CONFORMATION) Chain: V
Molecule details ›
Chain: V
Length: 26 nucleotides
Theoretical weight: 7.94 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P212121
Unit cell:
a: 52.194Å b: 63.653Å c: 130.205Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.212 0.264
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

14 review citations

Local and global regulation of transcription initiation in bacteria.
Browning et al. (2016)
13 more

11 mentions without citation

Bacterial Strategies to Maintain Zinc Metallostasis at the Host-Pathogen Interface.
Capdevila et al. (2016)
10 more