› 4wnn

X-ray diffraction
1.8Å resolution

SPT16-H2A-H2B FACT HISTONE Complex

Released:
DOI: 10.2210/pdb4wnn/pdb
PDB entry 4wnn coloured by chain, front view.
PDB entry 4wnn coloured by chain, side view.
PDB entry 4wnn coloured by chain, top view.
Source organisms:
Primary publication:
FACT Disrupts Nucleosome Structure by Binding H2A-H2B with Conserved Peptide Motifs.
Kemble DJ, McCullough LL, Whitby FG, Formosa T, Hill CP
Mol Cell 60 294-306 (2015)
PMID: 26455391

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-135448 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Histone H2A.1 Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 132 amino acids
Theoretical weight: 14.01 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P04911 (Residues: 1-132; Coverage: 100%)
Gene names: H2A1, HTA1, SPT11, YD9934.10, YDR225W
Sequence domains:
Structure domains: Histone, subunit A
Histone H2B.1 Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 102 amino acids
Theoretical weight: 11.38 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P02293 (Residues: 31-131; Coverage: 77%)
Gene names: H2B1, HTB1, SPT12, YD9934.09C, YDR224C
Sequence domains: Core histone H2A/H2B/H3/H4
Structure domains: Histone, subunit A
FACT complex subunit SPT16 Chain: T
Molecule details ›
Chain: T
Length: 20 amino acids
Theoretical weight: 2.26 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P32558 (Residues: 957-972; Coverage: 2%)
Gene names: CDC68, SPT16, SSF1, YGL207W

Ligands and Environments

1 bound ligand:
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: R3
Unit cell:
a: 109.667Å b: 109.667Å c: 187.47Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.181 0.179 0.225
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Born to run: control of transcription elongation by RNA polymerase II.
Chen et al. (2018)
14 more

14 mentions without citation

Histone chaperone networks shaping chromatin function.
Hammond et al. (2017)
13 more