› 4woj

X-ray diffraction
2.45Å resolution

Aspartate Semialdehyde Dehydrogenase from Francisella tularensis

Released:
DOI: 10.2210/pdb4woj/pdb
PDB entry 4woj coloured by chain, front view.
PDB entry 4woj coloured by chain, side view.
PDB entry 4woj coloured by chain, top view.
Source organism: Francisella tularensis subsp. tularensis
Primary publication:
Structure of aspartate b-semialdehyde dehydrogenase from Francisella tularensis
Mank NJ, Pote S, Majorek KA, Arnette AK, Klapper VG, Hurlburt BK, Chruszcz M
Acta Crystallogr F Struct Biol Commun 74 14-22 (2018)

Function and Biology Details

Reaction catalysed: 
L-aspartate 4-semialdehyde + phosphate + NADP(+) = L-4-aspartyl phosphate + NADPH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184462 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
aspartate-semialdehyde dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 370 amino acids
Theoretical weight: 41.42 KDa
Source organism: Francisella tularensis subsp. tularensis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8G8T7 (Residues: 1-364; Coverage: 100%)
Gene name: asd
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 3 x SO4
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P1
Unit cell:
a: 46.587Å b: 60.573Å c: 67.684Å
α: 115.43° β: 100.98° γ: 91.74°
R-values:
R R work R free
0.186 0.183 0.238
Expression system: Escherichia coli BL21(DE3)

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