› 4woq

X-ray diffraction
2.2Å resolution

Crystal Structures of CdNal from Clostridium difficile in complex with ketobutyric

Released:
DOI: 10.2210/pdb4woq/pdb
PDB entry 4woq coloured by chain, front view.
PDB entry 4woq coloured by chain, side view.
PDB entry 4woq coloured by chain, top view.
Source organism: Clostridioides difficile NAP08
Entry authors: Liu WD, Guo RT, Cui YF, Chen X, Wu QQ, Zhu DM

Function and Biology Details

Reaction catalysed: 
Aceneneuramate = N-acetyl-D-mannosamine + pyruvate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112718 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-acetylneuraminate lyase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 302 amino acids
Theoretical weight: 34.37 KDa
Source organism: Clostridioides difficile NAP08
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D5Q364 (Residues: 1-302; Coverage: 100%)
Gene names: HMPREF0220_1346, nanA
Sequence domains: Dihydrodipicolinate synthetase family
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:
Ligand 2KT 8 x 2KT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P21
Unit cell:
a: 81.875Å b: 122.612Å c: 122.186Å
α: 90° β: 95.72° γ: 90°
R-values:
R R work R free
0.184 0.184 0.243
Expression system: Escherichia coli BL21(DE3)

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