› 4wpz

X-ray diffraction
2.1Å resolution

Crystal structure of cytochrome P450 CYP107W1 from Streptomyces avermitilis

Released:
DOI: 10.2210/pdb4wpz/pdb
PDB entry 4wpz coloured by chain, front view.
PDB entry 4wpz coloured by chain, side view.
PDB entry 4wpz coloured by chain, top view.
Source organism: Streptomyces avermitilis
Primary publication:
Functional characterization of CYP107W1 from Streptomyces avermitilis and biosynthesis of macrolide oligomycin A.
Han S, Pham TV, Kim JH, Lim YR, Park HG, Cha GS, Yun CH, Chun YJ, Kang LW, Kim D
Arch Biochem Biophys 575 1-7 (2015)
PMID: 25849761

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188058 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P450 Chain: A
Molecule details ›
Chain: A
Length: 397 amino acids
Theoretical weight: 43.35 KDa
Source organism: Streptomyces avermitilis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q93HJ0 (Residues: 8-404; Coverage: 98%)
Gene names: SAV14893_021380, SAV31267_066180, olmB
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments

2 bound ligands:
Ligand K 2 x K
Ligand HEM 1 x HEM
1 modified residue:
Ligand CSD 1 x CSD

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: P43212
Unit cell:
a: 114.877Å b: 114.877Å c: 79.049Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.178 0.24
Expression system: Escherichia coli

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Citations

6 citation in other articles

Biochemical and Structural Characterization of MycCI, a Versatile P450 Biocatalyst from the Mycinamicin Biosynthetic Pathway.
DeMars et al. (2016)
5 more

5 mentions without citation

Recent Structural Insights into Cytochrome P450 Function.
Guengerich et al. (2016)
4 more