› 4wqd

X-ray diffraction
1.22Å resolution

Thiosulfate dehydrogenase (TsdA) from Allochromatium vinosum - K208G mutant

Released:
DOI: 10.2210/pdb4wqd/pdb
PDB entry 4wqd coloured by chain, front view.
PDB entry 4wqd coloured by chain, side view.
PDB entry 4wqd coloured by chain, top view.
Source organism: Allochromatium vinosum DSM 180
Primary publication:
Thiosulfate dehydrogenase (TsdA) from Allochromatium vinosum: structural and functional insights into thiosulfate oxidation.
Brito JA, Denkmann K, Pereira IA, Archer M, Dahl C
J Biol Chem 290 9222-38 (2015)
PMID: 25673691

Function and Biology Details

Reaction catalysed: 
2 thiosulfate + 2 ferricytochrome c = tetrathionate + 2 ferrocytochrome c
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112539 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thiosulfate dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 252 amino acids
Theoretical weight: 26.87 KDa
Source organism: Allochromatium vinosum DSM 180
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D3RVD4 (Residues: 29-270; Coverage: 100%)
Gene names: Alvin_0091, tsdA
Sequence domains:
Structure domains: Cytochrome c-like domain

Ligands and Environments


Cofactor: Ligand HEC 2 x HEC
4 bound ligands:
Ligand IOD 1 x IOD
Ligand EDO 1 x EDO
Ligand GAI 1 x GAI
Ligand SO4 1 x SO4
1 modified residue:
Ligand CSS 1 x CSS

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: C2
Unit cell:
a: 79.093Å b: 70.811Å c: 57.873Å
α: 90° β: 128.99° γ: 90°
R-values:
R R work R free
0.153 0.151 0.183
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Sulfur Oxidation in the Acidophilic Autotrophic Acidithiobacillus spp.
Wang et al. (2018)
2 more

1 mention without citation

Thiosulfate dehydrogenase (TsdA) from Allochromatium vinosum: structural and functional insights into thiosulfate oxidation.
Brito et al. (2015)