4wqk

X-ray diffraction
1.48Å resolution

Crystal structure of aminoglycoside nucleotidylyltransferase ANT(2")-Ia, apo form

Released:

Function and Biology Details

Reaction catalysed:
Nucleoside triphosphate + gentamicin = diphosphate + 2''-nucleotidylgentamicin
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-142393 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2''-aminoglycoside nucleotidyltransferase Chain: A
Molecule details ›
Chain: A
Length: 178 amino acids
Theoretical weight: 19.95 KDa
Source organism: Klebsiella pneumoniae
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AE05 (Residues: 1-177; Coverage: 100%)
Gene name: aadB
Sequence domains: Aminoglycoside-2''-adenylyltransferase
Structure domains: Beta Polymerase; domain 2

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 45.528Å b: 42.11Å c: 47.702Å
α: 90° β: 105.49° γ: 90°
R-values:
R R work R free
0.161 0.159 0.196
Expression system: Escherichia coli