› 4wr2

X-ray diffraction
1.7Å resolution

Crystal structure of a putative pyrimidine-specific ribonucleoside hydrolase (RihA) Protein from Shewanella loihica PV-4 (SHEW_0697, Target PSI-029635) with divalent cation and PEG 400 bound at the active site

Released:
DOI: 10.2210/pdb4wr2/pdb
PDB entry 4wr2 coloured by chain, front view.
PDB entry 4wr2 coloured by chain, side view.
PDB entry 4wr2 coloured by chain, top view.
Source organism: Shewanella loihica PV-4
Entry authors: Himmel DM, Bhosle R, Toro R, Hillerich B, Gizzi A, Garforth S, Kar A, Chan MK, Lafluer J, Patel H, Matikainen B, Chamala S, Lim S, Celikgil A, Villegas G, Evans B, Love J, Fiser A, Seidel RD, Bonanno JB, Almo SC, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107207 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyrimidine-specific ribonucleoside hydrolase RihA Chain: A
Molecule details ›
Chain: A
Length: 335 amino acids
Theoretical weight: 36.54 KDa
Source organism: Shewanella loihica PV-4
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A3QAS1 (Residues: 1-335; Coverage: 100%)
Gene names: Shew_0697, rihA
Sequence domains: Inosine-uridine preferring nucleoside hydrolase
Structure domains: Ribonucleoside hydrolase-like

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand 1PE 1 x 1PE
1 modified residue:
Ligand MSE 9 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: I222
Unit cell:
a: 60.078Å b: 115.047Å c: 141.64Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.157 0.157 0.171
Expression system: Escherichia coli BL21(DE3)

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