› 4wsn

X-ray diffraction
5.5Å resolution

Crystal structure of the COP9 signalosome, a P1 crystal form

Released:
DOI: 10.2210/pdb4wsn/pdb
PDB entry 4wsn coloured by chain, front view.
PDB entry 4wsn coloured by chain, side view.
PDB entry 4wsn coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cullin-RING ubiquitin E3 ligase regulation by the COP9 signalosome.
Cavadini S, Fischer ES, Bunker RD, Potenza A, Lingaraju GM, Goldie KN, Mohamed WI, Faty M, Petzold G, Beckwith RE, Tichkule RB, Hassiepen U, Abdulrahman W, Pantelic RS, Matsumoto S, Sugasawa K, Stahlberg H, Thomä NH
Nature 531 598-603 (2016)
PMID: 27029275

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158233 (preferred)
Entry contents:
8 distinct polypeptide molecules
Macromolecules (8 distinct):
COP9 signalosome complex subunit 1 Chains: A, I, Q, Y, g, o
Molecule details ›
Chains: A, I, Q, Y, g, o
Length: 480 amino acids
Theoretical weight: 54.22 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q13098 (Residues: 12-491; Coverage: 97%)
Gene names: COPS1, CSN1, GPS1
Sequence domains:
COP9 signalosome complex subunit 2 Chains: B, J, R, Z, h, p
Molecule details ›
Chains: B, J, R, Z, h, p
Length: 447 amino acids
Theoretical weight: 51.99 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P61201 (Residues: 1-443; Coverage: 100%)
Gene names: COPS2, CSN2, TRIP15
Sequence domains: PCI domain
COP9 signalosome complex subunit 3 Chains: C, K, S, a, i, q
Molecule details ›
Chains: C, K, S, a, i, q
Length: 427 amino acids
Theoretical weight: 48.25 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9UNS2 (Residues: 1-423; Coverage: 100%)
Gene names: COPS3, CSN3
Sequence domains:
COP9 signalosome complex subunit 4 Chains: D, L, T, b, j, r
Molecule details ›
Chains: D, L, T, b, j, r
Length: 410 amino acids
Theoretical weight: 46.65 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9BT78 (Residues: 1-406; Coverage: 100%)
Gene names: COPS4, CSN4
Sequence domains:
COP9 signalosome complex subunit 5 Chains: E, M, U, c, k, s
Molecule details ›
Chains: E, M, U, c, k, s
Length: 325 amino acids
Theoretical weight: 36.64 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q92905 (Residues: 14-334; Coverage: 96%)
Gene names: COPS5, CSN5, JAB1
Sequence domains:
COP9 signalosome complex subunit 6 Chains: F, N, V, d, l, t
Molecule details ›
Chains: F, N, V, d, l, t
Length: 331 amino acids
Theoretical weight: 36.53 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q7L5N1 (Residues: 1-327; Coverage: 100%)
Gene names: COPS6, CSN6, HVIP
Sequence domains:
COP9 signalosome complex subunit 7a Chains: G, O, W, e, m, u
Molecule details ›
Chains: G, O, W, e, m, u
Length: 222 amino acids
Theoretical weight: 24.65 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9UBW8 (Residues: 1-218; Coverage: 79%)
Gene names: COPS7A, CSN7A, DERP10
Sequence domains:
COP9 signalosome complex subunit 8 Chains: H, P, X, f, n, v
Molecule details ›
Chains: H, P, X, f, n, v
Length: 213 amino acids
Theoretical weight: 23.57 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q99627 (Residues: 1-209; Coverage: 100%)
Gene names: COPS8, CSN8
Sequence domains: CSN8/PSMD8/EIF3K family

Ligands and Environments

1 bound ligand:
Ligand ZN 6 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P1
Unit cell:
a: 150.638Å b: 150.983Å c: 336.72Å
α: 92.34° β: 92.62° γ: 119.88°
R-values:
R R work R free
0.255 0.254 0.282
Expression system: Trichoplusia ni

Quick links

Citations

30 review citations

Mechanisms of Deubiquitinase Specificity and Regulation.
Mevissen et al. (2017)
29 more

1 mention without citation

Structural basis of Cullin 2 RING E3 ligase regulation by the COP9 signalosome.
Faull et al. (2019)