› 4wt9

X-ray diffraction
2.5Å resolution

APO CRYSTAL STRUCTURE OF HCV NS5B GENOTYPE 2A JFH-1 ISOLATE WITH E86Q E87Q S15G C223H V321I AND DELTA8 MUTATIONS

Released:
DOI: 10.2210/pdb4wt9/pdb
Model geometry
Fit model/data
PDB entry 4wt9 coloured by chain, front view.
PDB entry 4wt9 coloured by chain, side view.
PDB entry 4wt9 coloured by chain, top view.
Source organism: Hepatitis C virus JFH-1
Primary publication:
Viral replication. Structural basis for RNA replication by the hepatitis C virus polymerase.
Appleby TC, Perry JK, Murakami E, Barauskas O, Feng J, Cho A, Fox D, Wetmore DR, McGrath ME, Ray AS, Sofia MJ, Swaminathan S, Edwards TE
Science 347 771-5 (2015)
PMID: 25678663

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Hydrolysis of four peptide bonds in the viral precursor polyprotein, commonly with Asp or Glu in the P6 position, Cys or Thr in P1 and Ser or Ala in P1'.
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189307 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-directed RNA polymerase Chain: A
Molecule details ›
Chain: A
Length: 572 amino acids
Theoretical weight: 63.76 KDa
Source organism: Hepatitis C virus JFH-1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q99IB8 (Residues: 2443-2885, 2896-3012; Coverage: 19%)
Sequence domains: Viral RNA dependent RNA polymerase

Ligands and Environments

3 bound ligands:
Ligand CL 1 x CL
Ligand EPE 1 x EPE
Ligand PG6 1 x PG6
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P65
Unit cell:
a: 140.37Å b: 140.37Å c: 92.23Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.195 0.193 0.245
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

53 review citations

Coronavirus RNA Proofreading: Molecular Basis and Therapeutic Targeting.
Robson et al. (2020)
52 more

1 mention without citation

NMR reveals the intrinsically disordered domain 2 of NS5A protein as an allosteric regulator of the hepatitis C virus RNA polymerase NS5B.
Bessa et al. (2017)