› 4wu2

X-ray diffraction
2.15Å resolution

Structure of the PTP-like myo-inositol phosphatase from Selenomonas ruminantium in complex with myo-inositol-(1,4,5)-trikisphosphate

Released:
DOI: 10.2210/pdb4wu2/pdb
PDB entry 4wu2 coloured by chain, front view.
PDB entry 4wu2 coloured by chain, side view.
PDB entry 4wu2 coloured by chain, top view.
Source organism: Selenomonas ruminantium
Primary publication:
Structure of the PTP-like phytase from Selenomonas ruminantium in complex with myo-inositol-(1,4,5)-trikisphosphate
Bruder LM
To be published

Function and Biology Details

Reaction catalysed: 
Myo-inositol hexakisphosphate + H(2)O = 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182145 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine specific protein phosphatases domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 342 amino acids
Theoretical weight: 39.13 KDa
Source organism: Selenomonas ruminantium
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7WUJ1 (Residues: 28-346; Coverage: 100%)
Gene name: phyA
Sequence domains: Inositol hexakisphosphate
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand I3P 2 x I3P
Ligand PO4 2 x PO4
Ligand GOL 4 x GOL
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P21
Unit cell:
a: 45.98Å b: 137.67Å c: 80.01Å
α: 90° β: 102.42° γ: 90°
R-values:
R R work R free
0.198 0.197 0.219
Expression system: Escherichia coli BL21(DE3)

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