› 4wuh

X-ray diffraction
2.29Å resolution

Crystal structure of E. faecalis DNA binding domain LiaR wild type complexed with 22bp DNA

Released:
DOI: 10.2210/pdb4wuh/pdb
PDB entry 4wuh coloured by chain, front view.
PDB entry 4wuh coloured by chain, side view.
PDB entry 4wuh coloured by chain, top view.
Source organism: Enterococcus faecalis S613
Primary publication:
A variable DNA recognition site organization establishes the LiaR-mediated cell envelope stress response of enterococci to daptomycin.
Davlieva M, Shi Y, Leonard PG, Johnson TA, Zianni MR, Arias CA, Ladbury JE, Shamoo Y
Nucleic Acids Res 43 4758-73 (2015)
PMID: 25897118

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-118234 (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
Response regulator receiver domain protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 68 amino acids
Theoretical weight: 7.71 KDa
Source organism: Enterococcus faecalis S613
Expression system: Escherichia coli BL21(DE3)
DNA (5'-D(P*GP*GP*AP*CP*TP*TP*AP*AP*GP*AP*AP*CP*GP*AP*TP*TP*T)-3') Chain: G
Molecule details ›
Chain: G
Length: 17 nucleotides
Theoretical weight: 5.25 KDa
Source organism: Enterococcus faecalis S613
Expression system: Not provided
DNA (5'-D(P*TP*TP*CP*TP*TP*AP*AP*GP*TP*CP*C)-3') Chain: H
Molecule details ›
Chain: H
Length: 11 nucleotides
Theoretical weight: 3.3 KDa
Source organism: Enterococcus faecalis S613
Expression system: Not provided
DNA (5'-D(P*AP*AP*AP*TP*CP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 6 nucleotides
Theoretical weight: 1.82 KDa
Source organism: Enterococcus faecalis S613
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P212121
Unit cell:
a: 38.332Å b: 77.932Å c: 104.696Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.215 0.259
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

6 review citations

Mechanisms of drug resistance: daptomycin resistance.
Tran et al. (2015)
5 more

3 mentions without citation

The structural and functional workings of KEOPS.
Beenstock et al. (2021)
2 more