› 4wuw

X-ray diffraction
2.4Å resolution

Crystal Structure of Surfactant Protein-A DED Mutant (E171D/P175E/K203D) Complexed with Inositol

Released:
DOI: 10.2210/pdb4wuw/pdb
PDB entry 4wuw coloured by chain, front view.
PDB entry 4wuw coloured by chain, side view.
PDB entry 4wuw coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Differential Ligand Binding Specificities of the Pulmonary Collectins Are Determined by the Conformational Freedom of a Surface Loop.
Rynkiewicz MJ, Wu H, Cafarella TR, Nikolaidis NM, Head JF, Seaton BA, McCormack FX
Biochemistry 56 4095-4105 (2017)
PMID: 28719181

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140125 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pulmonary surfactant-associated protein A Chain: A
Molecule details ›
Chain: A
Length: 148 amino acids
Theoretical weight: 16.71 KDa
Source organism: Rattus norvegicus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P08427 (Residues: 101-248; Coverage: 65%)
Gene names: Sftp-1, Sftp1, Sftpa, Sftpa1
Sequence domains: Lectin C-type domain
Structure domains: Mannose-Binding Protein A, subunit A

Ligands and Environments

2 bound ligands:
Ligand INS 1 x INS
Ligand CA 2 x CA
1 modified residue:
Ligand MHO 2 x MHO

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P63
Unit cell:
a: 97.699Å b: 97.699Å c: 45.027Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.177 0.174 0.204
Expression system: Trichoplusia ni

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Citations

1 review citation

The Role of Collectins and Galectins in Lung Innate Immune Defense.
Casals et al. (2018)
4 other articles