› 4ww4

X-ray diffraction
2.94Å resolution

Double-heterohexameric rings of full-length Rvb1(ADP)/Rvb2(ADP)

Released:
DOI: 10.2210/pdb4ww4/pdb
Model geometry
Fit model/data
PDB entry 4ww4 coloured by chain, front view.
PDB entry 4ww4 coloured by chain, side view.
PDB entry 4ww4 coloured by chain, top view.
Source organism: Thermochaetoides thermophila DSM 1495
Primary publication:
Structural basis for dodecameric assembly states and conformational plasticity of the full-length AAA+ ATPases Rvb1 · Rvb2.
Lakomek K, Stoehr G, Tosi A, Schmailzl M, Hopfner KP
Structure 23 483-495 (2015)
PMID: 25661652

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dodecamer
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123896 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
RuvB-like helicase Chain: A
Molecule details ›
Chain: A
Length: 462 amino acids
Theoretical weight: 50.45 KDa
Source organism: Thermochaetoides thermophila DSM 1495
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G0RYI5 (Residues: 1-462; Coverage: 100%)
Gene name: CTHT_0006820
Sequence domains:
RuvB-like helicase Chain: B
Molecule details ›
Chain: B
Length: 513 amino acids
Theoretical weight: 55.94 KDa
Source organism: Thermochaetoides thermophila DSM 1495
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G0RYC2 (Residues: 1-488; Coverage: 100%)
Gene name: CTHT_0006170
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ADP 2 x ADP
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID29
Spacegroup: R32
Unit cell:
a: 206.848Å b: 206.848Å c: 137.441Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.199 0.198 0.223
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

RPAP3 C-Terminal Domain: A Conserved Domain for the Assembly of R2TP Co-Chaperone Complexes.
Rodríguez et al. (2020)
2 more

8 mentions without citation

AAA ATPases as therapeutic targets: Structure, functions, and small-molecule inhibitors.
Zhang et al. (2021)
7 more