› 4wx0

X-ray diffraction
1.7Å resolution

UndA complexed with beta-hydroxydodecanoic acid

Released:
DOI: 10.2210/pdb4wx0/pdb
PDB entry 4wx0 coloured by chain, front view.
PDB entry 4wx0 coloured by chain, side view.
PDB entry 4wx0 coloured by chain, top view.
Source organism: Pseudomonas protegens Pf-5
Primary publication:
Microbial biosynthesis of medium-chain 1-alkenes by a nonheme iron oxidase.
Rui Z, Li X, Zhu X, Liu J, Domigan B, Barr I, Cate JH, Zhang W
Proc Natl Acad Sci U S A 111 18237-42 (2014)
PMID: 25489112

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175605 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
TENA/THI-4 family protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 263 amino acids
Theoretical weight: 30.35 KDa
Source organism: Pseudomonas protegens Pf-5
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4K8M0 (Residues: 1-261; Coverage: 100%)
Gene name: PFL_4321
Sequence domains: Iron-containing redox enzyme
Structure domains: Heme oxygenase-like

Ligands and Environments

4 bound ligands:
Ligand FE 2 x FE
Ligand HXD 2 x HXD
Ligand PER 2 x PER
Ligand SO4 8 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P2221
Unit cell:
a: 67.41Å b: 73.99Å c: 142.51Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.139 0.137 0.185
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Oxygen Activation and Radical Transformations in Heme Proteins and Metalloporphyrins.
Huang et al. (2018)
14 more

2 mentions without citation

Microbial biosynthesis of medium-chain 1-alkenes by a nonheme iron oxidase.
Rui et al. (2014)
1 more