› 4wxs

X-ray diffraction
1.9Å resolution

Crystal Structure of the E396D SNP Variant of the Myocilin Olfactomedin Domain

Released:
DOI: 10.2210/pdb4wxs/pdb
PDB entry 4wxs coloured by chain, front view.
PDB entry 4wxs coloured by chain, side view.
PDB entry 4wxs coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for misfolding in myocilin-associated glaucoma.
Donegan RK, Hill SE, Freeman DM, Nguyen E, Orwig SD, Turnage KC, Lieberman RL
Hum Mol Genet 24 2111-24 (2015)
PMID: 25524706

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189295 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Myocilin, C-terminal fragment Chain: A
Molecule details ›
Chain: A
Length: 277 amino acids
Theoretical weight: 31.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99972 (Residues: 228-504; Coverage: 59%)
Gene names: GLC1A, MYOC, TIGR
Sequence domains: Olfactomedin-like domain

Ligands and Environments

5 bound ligands:
Ligand CA 1 x CA
Ligand GOL 3 x GOL
Ligand PG4 1 x PG4
Ligand P6G 1 x P6G
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: I222
Unit cell:
a: 68.88Å b: 86.65Å c: 87.44Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.172 0.21
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Genetics of glaucoma.
Wiggs et al. (2017)
16 more

6 mentions without citation

Automated Structure- and Sequence-Based Design of Proteins for High Bacterial Expression and Stability.
Goldenzweig et al. (2016)
5 more