› 4wxw

X-ray diffraction
1.8Å resolution

SadC (323-487) from Pseudomonas aeruginosa PAO1

Released:
DOI: 10.2210/pdb4wxw/pdb
PDB entry 4wxw coloured by chain, front view.
PDB entry 4wxw coloured by chain, side view.
PDB entry 4wxw coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Entry authors: Liu C, Liu S, Gu L

Function and Biology Details

Reaction catalysed: 
2 GTP = 2 diphosphate + cyclic di-3',5'-guanylate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191046 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
diguanylate cyclase Chain: A
Molecule details ›
Chain: A
Length: 188 amino acids
Theoretical weight: 21.01 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HW69 (Residues: 323-487; Coverage: 34%)
Gene name: PA4332
Sequence domains: Diguanylate cyclase, GGDEF domain
Structure domains: Alpha-Beta Plaits

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BSRF BEAMLINE 3W1A
Spacegroup: P212121
Unit cell:
a: 50.913Å b: 51.102Å c: 54.59Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.185 0.217
Expression system: Escherichia coli BL21(DE3)

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