› 4wzp

X-ray diffraction
1.9Å resolution

Ser65 phosphorylated ubiquitin, major conformation

Released:
DOI: 10.2210/pdb4wzp/pdb
PDB entry 4wzp coloured by chain, front view.
PDB entry 4wzp coloured by chain, side view.
PDB entry 4wzp coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Ubiquitin Ser65 phosphorylation affects ubiquitin structure, chain assembly and hydrolysis.
Wauer T, Swatek KN, Wagstaff JL, Gladkova C, Pruneda JN, Michel MA, Gersch M, Johnson CM, Freund SM, Komander D
EMBO J 34 307-25 (2015)
PMID: 25527291

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143231 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 76 amino acids
Theoretical weight: 8.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CG47 (Residues: 153-228; Coverage: 33%)
Gene name: UBB
Sequence domains: Ubiquitin family

Ligands and Environments

1 bound ligand:
Ligand SO4 7 x SO4
1 modified residue:
Ligand SEP 8 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P1
Unit cell:
a: 49.563Å b: 53.285Å c: 56.866Å
α: 96.86° β: 104.94° γ: 110.96°
R-values:
R R work R free
0.196 0.193 0.238
Expression system: Escherichia coli

Quick links

Citations

82 review citations

Ubiquitin modifications.
Swatek et al. (2016)
81 more

13 mentions without citation

Specificity and disease in the ubiquitin system.
Chaugule et al. (2016)
12 more