› 4wzr

X-ray diffraction
2.15Å resolution

Crystal structure of human Puf-A

Released:
DOI: 10.2210/pdb4wzr/pdb
PDB entry 4wzr coloured by chain, front view.
PDB entry 4wzr coloured by chain, side view.
PDB entry 4wzr coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A divergent Pumilio repeat protein family for pre-rRNA processing and mRNA localization.
Qiu C, McCann KL, Wine RN, Baserga SJ, Hall TM
Proc Natl Acad Sci U S A 111 18554-9 (2014)
PMID: 25512524

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172276 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pumilio homolog 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 526 amino acids
Theoretical weight: 59.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15397 (Residues: 123-648; Coverage: 81%)
Gene names: KIAA0020, PUF-A, PUM3, XTP5, cPERP-C
Sequence domains: CPL (NUC119) domain

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2221
Unit cell:
a: 93.495Å b: 97.128Å c: 282.801Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.18 0.226
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Post-transcriptional Regulatory Functions of Mammalian Pumilio Proteins.
Goldstrohm et al. (2018)
2 more

4 mentions without citation

A divergent Pumilio repeat protein family for pre-rRNA processing and mRNA localization.
Qiu et al. (2014)
3 more