› 4x0g

X-ray diffraction
3.21Å resolution

Structure of Bsg25A binding with DNA

Released:
DOI: 10.2210/pdb4x0g/pdb
PDB entry 4x0g coloured by chain, front view.
PDB entry 4x0g coloured by chain, side view.
PDB entry 4x0g coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Common and distinct DNA-binding and regulatory activities of the BEN-solo transcription factor family.
Dai Q, Ren A, Westholm JO, Duan H, Patel DJ, Lai EC
Genes Dev 29 48-62 (2015)
PMID: 25561495

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118242 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Early boundary activity protein 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 109 amino acids
Theoretical weight: 12.14 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9VR17 (Residues: 250-358; Coverage: 30%)
Gene names: Bsg25A, CG12205, Elba1
Sequence domains: BEN domain
Structure domains: BEN domain
DNA (5'-D(*GP*TP*TP*CP*CP*AP*AP*TP*TP*GP*GP*AP*A)-3') Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 13 nucleotides
Theoretical weight: 3.99 KDa
Source organism: Drosophila melanogaster
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ACT 3 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P31
Unit cell:
a: 92.55Å b: 92.55Å c: 62.998Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.231 0.228 0.277
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

Notch signalling in context.
Bray SJ. (2016)
2 more

1 mention without citation

Common and distinct DNA-binding and regulatory activities of the BEN-solo transcription factor family.
Dai et al. (2015)