› 4x0o

X-ray diffraction
2.2Å resolution

Beta-ketoacyl-(acyl carrier protein) synthase III-2 (FabH2) from Vibrio cholerae soaked with Acetyl-CoA

Released:
DOI: 10.2210/pdb4x0o/pdb
PDB entry 4x0o coloured by chain, front view.
PDB entry 4x0o coloured by chain, side view.
PDB entry 4x0o coloured by chain, top view.
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Entry authors: Hou J, Chruszcz M, Zheng H, Cooper DR, Chordia MD, Zimmerman MD, Anderson WF, Minor W, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + malonyl-[acyl-carrier-protein] = acetoacetyl-[acyl-carrier-protein] + CoA + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
hetero dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-191949 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Beta-ketoacyl-[acyl-carrier-protein] synthase III 2 Chains: A, B, C, E, F, G, H
Molecule details ›
Chains: A, B, C, E, F, G, H
Length: 362 amino acids
Theoretical weight: 39.11 KDa
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9KLJ3 (Residues: 1-362; Coverage: 100%)
Gene names: VC_A0751, fabH2
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1
Beta-ketoacyl-[acyl-carrier-protein] synthase III 2 Chain: D
Molecule details ›
Chain: D
Length: 362 amino acids
Theoretical weight: 39.07 KDa
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9KLJ3 (Residues: 1-362; Coverage: 100%)
Gene names: VC_A0751, fabH2
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments


Cofactor: Ligand COA 7 x COA
2 bound ligands:
Ligand NA 7 x NA
Ligand MLI 1 x MLI
1 modified residue:
Ligand SCY 7 x SCY

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P1
Unit cell:
a: 61.763Å b: 86.98Å c: 157.342Å
α: 90.06° β: 89.96° γ: 90.08°
R-values:
R R work R free
0.175 0.174 0.201
Expression system: Escherichia coli BL21(DE3)

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